Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction
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چکیده
منابع مشابه
Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction.
Salmonella typhimurium definitive type 104 (DT104) is a virulent pathogen for humans and animals with many strains having multiple drug resistance characteristics. The organism typically carries resistance to ampicillin, chloramphenicol, florfenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT-resistant). A multiplex PCR method was developed to simultaneously amplify four genes, florfe...
متن کاملRapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction
BACKGROUND Infection with Salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. S. enterica serovar Typhimurium DT104 (DT104) strains are prevalent in livestock in Japan and include numerous strains of multidrug-resistant S. enterica. Epidemiological analysis of these strains is critical for both agricult...
متن کاملSpecific detection of Salmonella spp. by multiplex polymerase chain reaction.
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria. In addition to the phoP primers, the Hin and the H-1i primers, which tar...
متن کاملMultiplex polymerase chain reaction assay for selective detection of Salmonella enterica serovar typhimurium.
A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonel...
متن کاملSalmonella Typhimurium DT104, Italy
To the Editor: The recent article by Helms et al. described the distribution of Salmonella enterica serovar Typhimurium definitive page type 104 (DT104) infections in 29 countries from 1992 to 2001 (1). Results from Italy were not presented because routine phage typing was not performed before 2001. Since 2002, circulation of S. Typhimurium phage types has been monitored by the laboratorybased ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2000
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(99)00609-6